Detection of Antibiotic Resistance and Biofilm-Producing Ability of Staphylococcus Species in Clinical Isolates

Abstract Background Staphylococci are responsible for life-threatening infections in hospitals and community. Their ability to produce multiple virulence factors antibiotic resistance is an important reason of high mortality staphylococcal infections. Biofilm production by these organisms makes it difficult treat. Most the treating antibiotics failing making a matter concern. Aims This study aims detect increased biofilm-producing Staphylococcus compare performance three potential methods detection. Methods A total 81 isolates staphylococci including coagulase negative (CoNs), methicillin resistant S. aureus (MRSA), sensitive (MSSA) included this study. After identification, sensitivity test was performed. detection done different methods: Congo red agar method, tube adherence microtiter plate method. Result Out samples, 37 CoNs, 17 MRSA, 27 MSSA were identified. them we got 43 (53%) biofilm producers 40 (49%) 52 (64%) tissue culture plate/microtiter showed drug resistance. Conclusion We found out that method reliable as compared with other two methods. Antibiotic be very common producers. due developed result matrix does not let bind organisms. can make treatment future rate faster newly emerging antibiotics.